Biowulf 20th anniversary seminar series: Ryan Dale (NICHD)

The Biowulf 20th Anniversary Seminar Series continues with:

Integrative analyses of gene regulation via long-range chromatin 
interactions


Ryan Dale 
Scientific Information Officer and Bioinformatics Core Lead, NICHD

Tuesday, 9 April 2019
11 am - noon
Bldg 50, Rm 1227

This talk will be videocast at 
https://videocast.nih.gov/summary.asp?live=31795

Abstract:

Throughout development, gene expression is regulated in part by 
interactions between gene promoters and distal enhancers that can be 
tens or hundreds of kilobases away. Our recent work has identified novel 
mechanisms of such long-range regulation in fly and mouse models. In 
Drosophila, Argonaute2 is canonically part of the post-transcriptional 
siRNA silencing machinery. We show it also plays a role in transcription 
regulation via chromatin topology and RNA PolII phosphorylation though 
integrative analysis of RNA-seq, ChIP-seq, ChRIP-seq, and 4C-seq assays. 
In mouse, the erythroid transcription factor LDB1 is known to 
homodimerize to facilitate enhancer-promoter interactions, but by 
combining new data with published work we show that it can also interact 
with the architectural protein CTCF to promote the erythroid gene 
program in mouse. The software infrastructure that makes such 
integrative analyses possible -- and reproducible -- will also be 
highlighted.



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