NIH HPC News & Announcements
Reminder: Biowulf 20th anniversary seminar series: Ryan Dale (NICHD) TODAY Tuesday April 9
Date: 09 April 2019 08:04:56From: Susan Chacko
The Biowulf 20th Anniversary Seminar Series continues with: *Integrative analyses of gene regulation via long-range chromatin interactions* ** ** ***Ryan Dale (Scientific Information Officer and Bioinformatics Core Lead, NICHD) * *Tuesday, 9 April 2019* *11 am - noon* *Bldg 50, Rm 1227* This talk will be videocast at https://videocast.nih.gov/summary.asp?live=31795 *Abstract: * Throughout development, gene expression is regulated in part by interactions between gene promoters and distal enhancers that can be tens or hundreds of kilobases away. Our recent work has identified novel mechanisms of such long-range regulation in fly and mouse models. In Drosophila, Argonaute2 is canonically part of the post-transcriptional siRNA silencing machinery. We show it also plays a role in transcription regulation via chromatin topology and RNA PolII phosphorylation though integrative analysis of RNA-seq, ChIP-seq, ChRIP-seq, and 4C-seq assays. In mouse, the erythroid transcription factor LDB1 is known to homodimerize to facilitate enhancer-promoter interactions, but by combining new data with published work we show that it can also interact with the architectural protein CTCF to promote the erythroid gene program in mouse. The software infrastructure that makes such integrative analyses possible -- and reproducible -- will also be highlighted. ######################################################################## Please contact staff@hpc.nih.gov with any questions about the NIH HPC Systems[Last 12 months of HPC announcements]