NIH HPC News & Announcements
Biowulf seminar series: Dr. Carson Chow (NIDDK), Tuesday February 4, 11am - noon
Date: 27 January 2020 14:01:38
From: "Resch, Wolfgang [C]"
Please join us next week for the first talk in the 2020 Biowulf seminar
series:
The Kinetics of Gene Transcription
Carson Chow, Ph.D.
Senior Investigator, Laboratory of Biological Modelling
NIDDK
Tuesday, February 4, 2020
11am - noon
Building 50, Room 1227
Abstract:
Transcriptional regulation in metazoans occurs through long-range
genomic contacts between enhancers and promoters, and most genes are
transcribed in episodic "bursts" of RNA synthesis. Transcription can be
affected by perturbations to transcription factors and nuclear
architecture. To understand the relationship between these phenomena and
the dynamic regulation of genes in response to upstream signals, a wide
range of experimental methods are used from in vitro measurements of
gene expression in a large number of cells to live-cell RNA imaging in
single cells. A means to synthesize this data and dissect the mechanism
of gene transcription is to develop and fit a fully stochastic kinetic
model of transcription kinetics and RNA abundance. I will present
ongoing work of such a model that can explain a wide range of phenomena
including the dose response curve for hormone-induced gene induction and
the heterogeneity in protein levels in human tissue. Most recently, we
have applied the model to single cell RNA measurements of the entire
human genome to characterize the effect of promoter-enhancer contacts. I
will show how harnessing the full capabilities of Biowulf is required to
perform the computations on such a large number of genes.
This talk will be videocast at: https://videocast.nih.gov/watch=35803
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