*** Bismark methylation extractor version v0.16.0 *** Trying to determine the type of mapping from the SAM header line of file test_data_bismark_bt2.bam Treating file(s) as single-end data (as extracted from @PG line) Setting core usage to single-threaded (default). Consider using --multicore <int> to speed up the extraction process. Summarising Bismark methylation extractor parameters: =============================================================== Bismark single-end SAM format specified (default) Number of cores to be used: 1 Output will be written to the current directory ('/spin1/users/user/bismark_test') Writing result file containing methylation information for C in CpG context from the original top strand to CpG_OT_test_data_bismark_bt2.txt Writing result file containing methylation information for C in CpG context from the complementary to original top strand to CpG_CTOT_test_data_bismark_bt2.txt Writing result file containing methylation information for C in CpG context from the complementary to original bottom strand to CpG_CTOB_test_data_bismark_bt2.txt Writing result file containing methylation information for C in CpG context from the original bottom strand to CpG_OB_test_data_bismark_bt2.txt Writing result file containing methylation information for C in CHG context from the original top strand to CHG_OT_test_data_bismark_bt2.txt Writing result file containing methylation information for C in CHG context from the complementary to original top strand to CHG_CTOT_test_data_bismark_bt2.txt Writing result file containing methylation information for C in CHG context from the complementary to original bottom strand to CHG_CTOB_test_data_bismark_bt2.txt Writing result file containing methylation information for C in CHG context from the original bottom strand to CHG_OB_test_data_bismark_bt2.txt Writing result file containing methylation information for C in CHH context from the original top strand to CHH_OT_test_data_bismark_bt2.txt Writing result file containing methylation information for C in CHH context from the complementary to original top strand to CHH_CTOT_test_data_bismark_bt2.txt Writing result file containing methylation information for C in CHH context from the complementary to original bottom strand to CHH_CTOB_test_data_bismark_bt2.txt Writing result file containing methylation information for C in CHH context from the original bottom strand to CHH_OB_test_data_bismark_bt2.txt Now reading in Bismark result file test_data_bismark_bt2.bam skipping SAM header line: @HD VN:1.0 SO:unsorted skipping SAM header line: @SQ SN:chrX LN:155270560 skipping SAM header line: @SQ SN:chrY LN:59373566 skipping SAM header line: @PG ID:Bismark VN:v0.16.1 CL:"bismark XandY test_data.fastq" Processed 378 lines in total Total number of methylation call strings processed: 378 Final Cytosine Methylation Report ================================= Total number of C's analysed: 3258 Total methylated C's in CpG context: 88 Total methylated C's in CHG context: 4 Total methylated C's in CHH context: 13 Total C to T conversions in CpG context: 108 Total C to T conversions in CHG context: 777 Total C to T conversions in CHH context: 2268 C methylated in CpG context: 44.9% C methylated in CHG context: 0.5% C methylated in CHH context: 0.6% Determining maximum read length for M-Bias plot Maximum read length of Read 1: 50 Determining maximum read length for M-Bias plot Maximum read length of Read 1: 50 Deleting unused files ... CpG_OT_test_data_bismark_bt2.txt contains data -> kept CpG_CTOT_test_data_bismark_bt2.txt was empty -> deleted CpG_CTOB_test_data_bismark_bt2.txt was empty -> deleted CpG_OB_test_data_bismark_bt2.txt contains data -> kept CHG_OT_test_data_bismark_bt2.txt contains data -> kept CHG_CTOT_test_data_bismark_bt2.txt was empty -> deleted CHG_CTOB_test_data_bismark_bt2.txt was empty -> deleted CHG_OB_test_data_bismark_bt2.txt contains data -> kept CHH_OT_test_data_bismark_bt2.txt contains data -> kept CHH_CTOT_test_data_bismark_bt2.txt was empty -> deleted CHH_CTOB_test_data_bismark_bt2.txt was empty -> deleted CHH_OB_test_data_bismark_bt2.txt contains data -> kept