In the previous Data Storage hands-on section, you should have copied the class scripts to your /data area. If you skipped or missed that section, type
hpc-classes biowulfnow. This command will copy the scripts and input files used in this online class to your /data area, and will take about 5 minutes.
In the following session, you will submit a swarm of Blat jobs, a sequence alignment program . If you're not familiar with this program, don't worry -- this is just an example. The basic principles of job submission are not specific for Blat.
cd /data/$USER/hpc-classes/biowulf/swarm # look at the blat.swarm file more blat.swarm # see how many lines (commands) are in the blat.swarm file, not including the comment lines grep -v '#' blat.swarm | wc -l # submit the swarm swarm -f blat.swarm --module blat
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[+] Loading BLAT 3.5then they all ran successfully. (Unfortunately, the 'module load blat' command puts its output into standard error, so all the .e files will have this line.) If one has a larger size, take a look at that file.
Most of the files in the 'out' directory will show only the Blat header. This means that no match was found for that query sequence against human genome chromosome 10. 'ls -l out' will show you which output files are larger. Those are the ones corresponding to query sequences which found matches in chromosome 10.