A comprehensive, accurate and efficient solution for analysis of large scale base-resolution DNA methylation data, bisulfite sequencing or single molecule direct sequencing.
Write a configuration file as described in the moabs manual and set up a batch script along the following lines:
#!/bin/bash # file called myjob.bat module load moabs cd /path/to/moabs/data moabs -cf myrun.cfg
Submit this job with:
[user@biowulf ~]$ sbatch myjob.bat
Set up a swarm command file containing one line for each of your MOABS runs. Typically, only the input sequence name will change from line to line, but in the example below, different parameters are being applied to each sequence.
Sample swarm command file
# --------file myjobs.swarm---------- moabs -cf myrun1.cfg moabs -cf myrun2.cfg moabs -cf myrun3.cfg .... moabs -cf myrunN.cfg # -----------------------------------
Submit this set of runs to the batch system by typing
[user@biowulf ~]$ swarm --module moabs -f myjobs.swarm
For details on using swarm see Swarm on Biowulf.