RGT on HPC
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Interactive job
Batch job
Swarm of jobs

HINT (Hmm-based IdeNtification of Transcription factor footprints) integrates both DNase I hypersensitivity and histone modifications for the detection of open chromatin regions and active binding sites. Within transcription factor binding sites, there is a specific grammar of DNase I digestion and histone marks. The authors have therefore devised a multivariate HMM to model this regulatory grammar by simultaneous analysis of DNase-seq and the ChIP-seq profiles of histone modifications on a genome-wide level. The HMM has as input a normalized and a slope signal of DNase-seq and one of the histone marks. It can therefore detect the increase, top and decrease regions of either histone modification and DNase signals. The genomic regions annotated with the HMM state are considered predictions and represent likely binding sites within that cell context. For benchmarking data of main publication please visit authors lab's website.

References:

Documentation
Important Notes

Interactive job
Interactive jobs should be used for debugging, graphics, or applications that cannot be run as batch jobs.

Allocate an interactive session and run the program. Sample session:

[user@biowulf]$ sinteractive --mem=10g
salloc.exe: Pending job allocation 46116226
salloc.exe: job 46116226 queued and waiting for resources
salloc.exe: job 46116226 has been allocated resources
salloc.exe: Granted job allocation 46116226
salloc.exe: Waiting for resource configuration
salloc.exe: Nodes cn3144 are ready for job

[user@cn3144 ~]$ module load rgt
[user@cn3144 ~]$ rgt-hint footprinting --dnase-seq DNase.bam DNasePeaks.bed

[user@cn3144 ~]$ exit
salloc.exe: Relinquishing job allocation 46116226
[user@biowulf ~]$

Batch job
Most jobs should be run as batch jobs.

Create a batch input file (e.g. batch.sh). For example:

#!/bin/bash
#SBATCH --job-name="script"
#SBATCH --mail-type=BEGIN,END

cp -p /usr/local/apps/rgt/DendriticCells.tar.gz /data/$USER/rgt
tar xvfz /data/$USER/rgt/DendriticCells
cd /data/$USER/rgt/DendriticCells 

module load rgt/0.13.2

rgt-hint footprinting --atac-seq --paired-end --organism=mm10 --output-location=./ --output-prefix=pDC pDC.bam pDC_peaks.narrowPeak

rgt-hint footprinting --atac-seq --paired-end --organism=mm10 --output-location=./ --output-prefix=cDC1 cDC1.bam cDC1_peaks.narrowPeak 

rgt-hint tracks --bc --bigWig --organism=mm10 cDC1.bam cDC1_peaks.narrowPeak  --output-prefix=cDC1_BC
rgt-hint tracks --bc --bigWig --organism=mm10 pDC.bam pDC_peaks.narrowPeak  --output-prefix=pDC_BC

rgt-motifanalysis matching --organism=mm10 --input-files pDC.bed cDC1.bed
rgt-hint differential --organism=mm10 --bc --nc $SLURM_CPUS_PER_TASK --mpbs-files=./match/cDC1_mpbs.bed,./match/pDC_mpbs.bed --reads-files=cDC1.bam,pDC.bam --conditions=cDC1,pDC --output-location=cDC1_pDC

Submit this job using the Slurm sbatch command. The $SLURM_CPUS_PER_TASK in the script will be replaced with the actual number of cpus automatically.

sbatch --cpus-per-task=16 --mem=25g --time=10:00:00 script
Swarm of Jobs
A swarm of jobs is an easy way to submit a set of independent commands requiring identical resources.

Create a swarmfile (e.g. job.swarm). For example:

cd dir1; rgt-hint footprinting --dnase-seq DNase.bam DNasePeaks.bed
cd dir2; rgt-hint footprinting --dnase-seq DNase.bam DNasePeaks.bed
cd dir3; rgt-hint footprinting --dnase-seq DNase.bam DNasePeaks.bed

Submit this job using the swarm command.

swarm -f job.swarm [-g #] [-t #] --module rgt
where
-g # Number of Gigabytes of memory required for each process (1 line in the swarm command file)
-t # Number of cpus required for each process (1 line in the swarm command file)
--module Loads the module for each subjob in the swarm