The NIH HPC group plans, manages and supports high-performance computing systems specifically for use by the intramural NIH community. These systems include Biowulf, a 105,000+ processor Linux cluster; Helix, an interactive system for file transfer and management, and Helixweb, which provides a number of web-based scientific tools. We provide access to a wide range of computational applications for genomics, molecular and structural biology, mathematical and graphical analysis, image analysis, and other scientific fields.

The continued growth and support of NIH's Biowulf cluster is dependent upon its demonstrable value to the NIH Intramural Research Program. If you publish research that involved significant use of Biowulf, please cite the cluster. Suggested citation text:

This work utilized the computational resources of the NIH HPC Biowulf cluster (https://hpc.nih.gov).

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Biowulf Utilization
Monday, March 17th, 2025
utilization graph
Last 24 hrs
88,715 jobs submitted
63,908 jobs completed
2,964,440 CPU hrs used
22 NIH Institutes
198 Principal Investigators
365 users

Announcements
(All)
Recent Papers that used Biowulf & HPC Resources
(All 6345 papers)

Structure of blood cell-specific tubulin and demonstration of dimer spacing compaction in a single protofilament
Montecinos, F; Eren, E; Watts, NR et al.
J Biol Chem , DOI://10.1016/j.jbc.2024.108132 (2025)


N-terminal fragment shedding contributes to signaling of the full-length adhesion receptor ADGRL3
Perry-Hauser, NA; Du Rand, JR; Lee, KH et al.
J Biol Chem , DOI://10.1016/j.jbc.2025.108174 (2025)


RNA Pol-II transcripts in nucleolar associated domains of cancer cell nucleoli
Chowdhury, SR; Shilpi, A; Felsenfeld, G; ,
Nucleus , DOI://10.1080/19491034.2025.2468597 (2025)


thumbnail image from paper The c.119-123dup5bp mutation in human γC-crystallin destabilizes the protein and activates the unfolded protein response to cause highly variable cataracts
Shah, MH; Vendra, VPR; Ostrowski, C et al.
Sci Rep , DOI://10.1038/s41598-025-90977-2 (2025)


Nitric oxide inhibits ten-eleven translocation DNA demethylases to regulate 5mC and 5hmC across the genome
Palczewski, MB; Kuschman, HP; Hoffman, BM et al.
Nat Commun , DOI://10.1038/s41467-025-56928-1 (2025)


Gut microbiome shifts in adolescents after sleeve gastrectomy with increased oral-associated taxa and pro-inflammatory potential
Akagbosu, CO; McCauley, KE; Namasivayam, S et al.
Gut Microbes , DOI://10.1080/19490976.2025.2467833 (2025)


ProtPipe: A Multifunctional Data Analysis Pipeline for Proteomics and Peptidomics
Li, Z; Weller, CA; Shah, S et al.
Genomics Proteomics Bioinformatics , DOI://10.1093/gpbjnl/qzae083 (2025)


Estimation of mosaic loss of Y chromosome cell fraction with genotyping arrays lacking coverage in the pseudoautosomal region
Zhou, W; Huang, WY; Freedman, ND; Machiela, M; ,
BMC Bioinformatics , DOI://10.1186/s12859-025-06076-6 (2025)