Biowulf High Performance Computing at the NIH
MEGAN on Biowulf

MEGAN is a MEtaGenome ANalyzer. It can read Blast output. Normally this program would be run on your own desktop, but MEGAN is a memory hog, and some desktop systems may not have enough memory to handle a large dataset.

MEGAN was developed by D. H. Huson et al at U. Tübingen.

MEGAN6 is a comprehensive toolbox for interactively analyzing microbiome data. All the interactive tools you need in one application.
  • Taxonomic analysis using the NCBI taxonomy or a customized taxonomy such as SILVA
  • Functional analysis using InterPro2GO, SEED, eggNOG or KEGG
  • Bar charts, word clouds, Voronoi tree maps and many other charts
  • PCoA, clustering and networks
  • Supports metadata
  • MEGAN parses many different types of input


Important Notes

This application requires a graphical connection using NX

Interactive job
Interactive jobs should be used for debugging, graphics, or applications that cannot be run as batch jobs.
Allocate an interactive node and run MEGAN there. Sample session requesting an allocation of 10 GB of RAM:
[user@biowulf ~]$ sinteractive --mem=10g
salloc.exe: Granted job allocation 143361

[user@cn0124 ~]$ module load MEGAN

[user@cn0124 ~]$ MEGAN

[user@cn0124 ~]$ exit exit srun: error: cn0124: task 0: Exited with exit code 130 salloc.exe: Relinquishing job allocation 143361 [user@biowulf ~]$