Fastqtools on HPC
A collection of small and efficient programs for performing some common and uncommon tasks with FASTQ files.
Documentation
- Module Name: fastqtools (see the modules page for more information)
Interactive job
Interactive jobs should be used for debugging, graphics, or applications that cannot be run as batch jobs.
Allocate an interactive session and run the program. Sample session:
[user@biowulf]$ sinteractive salloc.exe: Pending job allocation 46116226 salloc.exe: job 46116226 queued and waiting for resources salloc.exe: job 46116226 has been allocated resources salloc.exe: Granted job allocation 46116226 salloc.exe: Waiting for resource configuration salloc.exe: Nodes cn3144 are ready for job [user@cn3144 ~]$ module load fastqtools [user@cn3144 ~]$ fastq-unique input.fq > outfile [user@cn3144 ~]$ exit salloc.exe: Relinquishing job allocation 46116226 [user@biowulf ~]$
Batch job
Most jobs should be run as batch jobs.
Create a batch input file (e.g. batch.sh). For example:
#!/bin/bash set -e module load fastqtools fastq-unique input.fq > outfile
Submit this job using the Slurm sbatch command.
sbatch [--cpus-per-task=#] [--mem=#] batch.sh
Swarm of Jobs
A swarm of jobs is an easy way to submit a set of independent commands requiring identical resources.
Create a swarmfile (e.g. job.swarm). For example:
cd dir1; fastq-unique input.fq > outfile cd dir2; fastq-unique input.fq > outfile cd dir3; fastq-unique input.fq > outfile
Submit this job using the swarm command.
swarm -f job.swarm [-g #]--module fastqtoolswhere
-g # | Number of Gigabytes of memory required for each process (1 line in the swarm command file) |
--module | Loads the module for each subjob in the swarm |