MUSIC is an algorithm for identification of enriched regions at multiple scales in the read depth signals from ChIP-Seq experiments.

• Music Main Site

• Module Name: music (see the modules page for more information)
• Singlethreaded app

Allocate an interactive session and run the program. Sample session:

[user@biowulf]$ sinteractive
salloc.exe: Pending job allocation 46116226
salloc.exe: job 46116226 queued and waiting for resources
salloc.exe: job 46116226 has been allocated resources
salloc.exe: Granted job allocation 46116226
salloc.exe: Waiting for resource configuration
salloc.exe: Nodes cn3144 are ready for job

[user@cn3144 ~]$ module load music

[user@cn3144 ~]$ mkdir chip

[user@cn3144 ~]$ samtools view /usr/local/apps/macs/TEST_DATA/ENCFF001NGB.bam | MUSIC -preprocess SAM stdin chip/
Preprocessing:
Input Format: SAM
ChIP-Seq input file path: stdin
Parsed Reads Output Directory: chip/
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MUSIC finished preprocessing SAM formatted reads in 59 seconds.

[user@cn3144 ~]$ exit
salloc.exe: Relinquishing job allocation 46116226
[user@biowulf ~]$

Create a batch input file (e.g. music.sh). For example:

#!/bin/bash
set -e
module load music
samtools view 1.bam | MUSIC -preprocess SAM stdin chip/

Submit this job using the Slurm sbatch command.

sbatch music.sh

Create a swarmfile (e.g. music.swarm). For example:

samtools view 1.bam | MUSIC -preprocess SAM stdin chip1/
samtools view 2.bam | MUSIC -preprocess SAM stdin chip2/
samtools view 3.bam | MUSIC -preprocess SAM stdin chip3/

Submit this job using the swarm command.

swarm -f music.swarm --module music