Biowulf High Performance Computing at the NIH
UROPA on Biowulf

UROPA is a command line based tool intended for genomic region annotation.


Important Notes

Interactive job
Interactive jobs should be used for debugging, graphics, or applications that cannot be run as batch jobs.

Allocate an interactive session and run the program.
Sample session (user input in bold):

[user@biowulf]$ sinteractive
salloc.exe: Pending job allocation 46116226
salloc.exe: job 46116226 queued and waiting for resources
salloc.exe: job 46116226 has been allocated resources
salloc.exe: Granted job allocation 46116226
salloc.exe: Waiting for resource configuration
salloc.exe: Nodes cn3144 are ready for job

[user@cn1234 ~]$ module load uropa

[user@cn1234 ~]$ uropa -h
[+] Loading singularity  on cn3397 
[+] Loading uropa 2.0.3  ... 
[user@cn1234 ~]$ uropa -i test.json -p output/test -s
[user@cn1234 ~]$ exit
salloc.exe: Relinquishing job allocation 46116226
[user@biowulf ~]$

Batch job
Most jobs should be run as batch jobs.

Create a batch input file (e.g. For example:

set -e
module load uropa
uropa -i test.json -p output/test_gem -s

Submit this job using the Slurm sbatch command.

sbatch [--cpus-per-task=#] [--mem=#]
Swarm of Jobs
A swarm of jobs is an easy way to submit a set of independent commands requiring identical resources.

Create a swarmfile (e.g. uropa.swarm). For example:

uropa -i test1.json -p output/test1_gem -s
uropa -i test2.json -p output/test2_gem -s
uropa -i test3.json -p output/test3_gem -s

Submit this job using the swarm command.

swarm -f uropa.swarm [-g #] [-t #] --module uropa
-g # Number of Gigabytes of memory required for each process (1 line in the swarm command file)
-t # Number of threads/CPUs required for each process (1 line in the swarm command file).
--module uropa Loads the uropa module for each subjob in the swarm